When diagnosing bloodstream infection (BI) the tradition method could be the foundation for development of microorganisms and getting the bloodstream culture. Pancreatic digest from fish dinner may be the foundation of all culture media in Russia. In countries in europe brain-heart media (BHM) are utilized for finding microorganisms in blood. In Russia BHM is not produced. The target is to workout the formulation therefore the means of the BHM (broth and agar) planning to be able to improve the effectiveness of obtaining blood tradition. There have been defined the actual and chemical indices and biological variables of the BHM. The microbiological control of the BHM had been completed by diagnostic research of cardiological patients’ blood. On the basis of the developed means of the brain-heart removal (BHE) preparation there clearly was created the fluid and agar BHM (LBHM, BHA). The LBHM was poured into containers which then had been full of the inert fuel. The bottles were shut with rubber stoppers and rolled in metal limits became a closed system. Microbiological qan in MPA, (p less then 0,001). Anaerobic microbes would not develop in MPA. Whenever diagnosing bloodstream infection the BHM (liquid and agar) have the ability to create the ideal circumstances for the rise of the variety pathogen growth.Were studied the genes encoding the virulence factors of 221 strains E. coli O6H1 (194) and E. coli O25H4 (27), separated in 2014-2018 from stool types of kids and adults examined based on epidemic indications. Molecular methods included PCR with hybridization-fluorescence and electrophoresis detection of increased items. The strains did not have virulence genetics for diarrheagenic E. coli (DEC) pathogroups EPEC, ETEC, EIEC, EHEC, EAggEC, and belonged into the phylogenetic group B2. They included from four to eight genetics encoding virulence facets of ExPEC E. coli O6H1 – pap (68,6%), sfa (87,6%), fimH (96,4%), hly (62,4%), cnf (74,7%), iutA (97,9%), fyuA (95,9%), chu (100%); E. coli O25H4 – pap (66,7%), afa (22,2%), fimH (100%), hly (44,4%), cnf (44,4%), iutA (100%) , fyuA (100%), chu (100%). The antimicrobial susceptibility assessment to 6 courses of antimicrobials (beta-lactams, fluoroquinolones, aminoglycosides, nitrofurantoin, sulfanilamide, trimethoprim / sulfamethoxazole) according the EUCAST. 60,3% of E. coli O6H1 were sensitive to antibiotics, E. coli O25H4 remained sensitive to carbapenems and nitrofurans. Extended-spectrum cephalosporins resistance ended up being as a result of insurance medicine manufacturing ESBL (CTX-M). The 57,1% resistant strains of E. coli O6H1 and 100% of E. coli O25H4 strains belonged into the MDR phenotype. The XDR phenotype had one out of five MDR strains of E. coli O6H1 and E. coli O25H4. All strains of E. coli O25H4 belonged to ST131. Given the crucial part of E. coli in human pathology, recognition of virulence genetics is done to confirm the etiological importance of the isolated strain.A new original domestic pair of reagents happens to be developed when it comes to dedication of class G immunoglobulins to individual real human herpes simplex virus antigens of kind 7 because of the approach to immune blotting within the “Western-blot” structure. Preliminary clinical studies were conducted using 134 serums of healthy children elderly 1-16 years just who underwent diagnostic evaluating. Stud y of diagnostic effectiveness ofthe brand new system revealed large sensitivity, much like the susceptibility regarding the response indirect immunofluorescence and large specificity, which can be manifested within the lack of untrue excellent results whenever examination samples containing immunoglobulin G to herpes virus 6 type.The serum of 1006 children aged 0-18 years and elderly people aged 60 to 90 years and older for the existence of certain class G immunoglobulins into the Epstein-Barr virus was examined using enzyme immunoassay. The dependence of seropositivity of kids on their age and seropositivity in excess of 98% of most surveyed elderly people is shown.Comparative analysis of serum concentrations of chorionic gonadotropin (hCG) associated with all the pregnancy of plasma necessary protein A (PAPP-A) and alpha-fetoprotein (AFP), in line with the results of a survey of females as part of a standard assessment system (the outcome were expressed as a MoM – multiply of this median), found a significant escalation in the performance of all the studied specific pregnancy proteins in women with a scar in the uterus and placenta acctera (75 patients) weighed against the data of the band of expectant mothers without scar regarding the womb and without abnormalities of attachment regarding the placenta (150 ladies). AFP indices were 1.68 ± 0.76 and 1.19 ± 0.43 MoM (p = 0.0018), hCG – 1.62 ± 1.48 and 1.23 ± 0.76 mother (p = 0, 0112), PAPP-A – 1.93 ± 1.24 and 1.23 ± 0.67 mother (p less then 0.0001). Using the ROC analysis, the diagnostic thresholds for the concentrations of AFP, hCG and PAPP-A had been calculated. The risk of placenta accreta in women with a scar in the uterus in instances of exceeding the diagnostic limit of AFP concentration (1.64 MoM) increased 2.5 times (RR = 2.5; 95% CI 1.17-5.36, p = 0, 0185), hCG (1.41 mother) – 1.6 times (RR = 1.59; 95% CI 1.09-2.32, p = 0.0147), PAPP-A (1.41 MoM) – 2.65 times (RR = 2.65; 95% CI 1.76-3.99, p less then 0.0001). Determination associated with the standard of specific pregnancy proteins can be utilized when you look at the system of complex forecast of placental growth in pregnant women with a scar regarding the uterus as an addition towards the evaluation of medical and anamnestic risk elements.Results of comparative ELISA investigation of pretreatment sPD-1 and sPD-L1 content in blood plasma of 100 gastric cancer clients at various condition phases aged 25 to 81 many years are presented. Control group included 60 almost healthier donors elderly 18 – 68 years.